This project will investigate the potential for perfusion and stem cells to regenerate and repair and/or rejuvenate organs through the application of cellular therapies or their exosomes prior to transplantation. Amniotic membrane epithelial cells, which have shown great potential in eliciting protective and regenerative effects in lungs via anti-inflammatory, immunosuppressive and anti-fibrotic mechanisms, will be investigated in transplantation; including the lungs, liver, and kidneys.